Custom layout doesn't save across session

After creating a new layout “My Layout”, when I quit Blender and then restart, “My Layout” is no longer an option to be selected. Note that I have saved as a new startup file. What am I missing?

I have never really messed with creating a “Layout” per se since I change my layout at the Blender opening screen. But, my guess is you may not have saved your User Settings in the User Preference box before saving your new Start Up File. Sometimes Blender is just plain quirky and you have to try a couple of times to get it to work right.

Suggestion…Create a New Layout…change 1 or 2 things…Save User Settings in User Preferences…then save new Starter File. Close Blender then reopen to see if changes took. If they did, go to your layout and set up the rest of your settings saving again at User Preferences when done and save as a new startup file.

Hope this resolves your issue.

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Thanks. I see it now. New screens are only saved in the start up file.

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